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Structured Review

TransGen biotech co pet30a-aahd1
Pet30a Aahd1, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a-aahd1/pet+30a/pm33165526-85-6-21
Average 90 stars, based on 1 article reviews
pet30a-aahd1 - by Bioz Stars, 2026-09
90/100 stars

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Expressing:

Article Title: The WRKY transcription factor AaGSW2 promotes glandular trichome initiation in Artemisia annua.
Article Snippet: For protein expression and purification, the vectors PET30a-AaHD1, PET30a-AaHD8, and empty PET-30a were separately transformed into Escherichia coli strain Rosetta (DE3) (TransGen Biotech, China) and heterologous protein production was induced by adding 0.5 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG) to the bacterial cultures at 16 °C and incubating for 16 h. The proteins were purified using HisSep Ni-NTA (nitrilotriacetic) agarose resin (Yeasen, China) according to the manufacturer’s instructions.

Purification:

Article Title: The WRKY transcription factor AaGSW2 promotes glandular trichome initiation in Artemisia annua.
Article Snippet: For protein expression and purification, the vectors PET30a-AaHD1, PET30a-AaHD8, and empty PET-30a were separately transformed into Escherichia coli strain Rosetta (DE3) (TransGen Biotech, China) and heterologous protein production was induced by adding 0.5 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG) to the bacterial cultures at 16 °C and incubating for 16 h. The proteins were purified using HisSep Ni-NTA (nitrilotriacetic) agarose resin (Yeasen, China) according to the manufacturer’s instructions.

Positron Emission Tomography:

Article Title: The WRKY transcription factor AaGSW2 promotes glandular trichome initiation in Artemisia annua.
Article Snippet: For protein expression and purification, the vectors PET30a-AaHD1, PET30a-AaHD8, and empty PET-30a were separately transformed into Escherichia coli strain Rosetta (DE3) (TransGen Biotech, China) and heterologous protein production was induced by adding 0.5 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG) to the bacterial cultures at 16 °C and incubating for 16 h. The proteins were purified using HisSep Ni-NTA (nitrilotriacetic) agarose resin (Yeasen, China) according to the manufacturer’s instructions.

Transformation Assay:

Article Title: The WRKY transcription factor AaGSW2 promotes glandular trichome initiation in Artemisia annua.
Article Snippet: For protein expression and purification, the vectors PET30a-AaHD1, PET30a-AaHD8, and empty PET-30a were separately transformed into Escherichia coli strain Rosetta (DE3) (TransGen Biotech, China) and heterologous protein production was induced by adding 0.5 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG) to the bacterial cultures at 16 °C and incubating for 16 h. The proteins were purified using HisSep Ni-NTA (nitrilotriacetic) agarose resin (Yeasen, China) according to the manufacturer’s instructions.



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TransGen biotech co pet30a-aahd1
Pet30a Aahd1, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a-aahd1/pet+30a/pm33165526-85-6-21
Average 90 stars, based on 1 article reviews
pet30a-aahd1 - by Bioz Stars, 2026-09
90/100 stars
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